pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)

LOCUS       pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)               12891 bp ds-DNA     circular SYN 08-FEB-2023
DEFINITION  Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate 
            design(c+iii).
ACCESSION   .
VERSION     .
KEYWORDS    pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 12891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 8, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      5..140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     67..86
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          1288..1353
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1483..1503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1928..2062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1965..1984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2019..2038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2230..2863
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2910..3035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3532..3765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3950..3994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4279..4396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4470..5648
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4701..5639
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5596..5616
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5685..5694
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6480..6521
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7026..7082
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8214..8924
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8359..8377)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8627..8646
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8984..9572
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9037..9057)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9455..9466)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9575..9591)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9576..9592)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9682..9915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9940..9958)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9941..9958)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10248..10267)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10481..10500)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10600..10622
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10660..10678)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10746..10850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10851..11711
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11069..11088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11882..12470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12371..12390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12624..12641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12711..12731)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12716..154
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
       61 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      121 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
      181 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
      241 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
      301 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
      361 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
      421 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
      481 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
      541 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
      601 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
      661 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
      721 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
      781 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
      841 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
      901 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
      961 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     1021 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     1081 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     1141 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     1201 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     1261 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     1321 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     1381 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     1441 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     1501 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     1561 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     1621 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     1681 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     1741 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     1801 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     1861 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     1921 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2041 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     2101 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     2161 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     2221 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     2281 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     2341 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     2401 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     2461 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     2521 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     2581 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     2641 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     2701 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2761 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2821 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2881 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2941 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     3001 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     3061 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     3121 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     3181 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3241 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3301 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3361 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3421 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3481 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3541 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3601 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3661 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3721 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3781 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3841 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3901 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3961 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     4021 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     4081 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     4141 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     4201 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
     4261 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4321 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4381 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
     4441 gcttgatatc gaattcctgc agcccgtgag gctccggtgc ccgtcagtgg gcagagcgca
     4501 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     4561 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     4621 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     4681 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     4741 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     4801 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     4861 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     4921 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     4981 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     5041 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     5101 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     5161 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     5221 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     5281 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     5341 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     5401 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     5461 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     5521 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     5581 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     5641 tgtcgtgagg ggatctggag ctctcgagaa ttctcacgcg tgccgccacc atggccttac
     5701 cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg ccggacatcc
     5761 agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc accatcagtt
     5821 gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa ccagatggaa
     5881 ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca tcaaggttca
     5941 gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag caagaagata
     6001 ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga ggcggcacca
     6061 agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc ggcggatctg
     6121 aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc ctgtccgtca
     6181 catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt cgccagcctc
     6241 cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca tactataatt
     6301 cagctctcaa atccagactg accatcatca aggacaactc caagagccaa gttttcttaa
     6361 aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa cattattact
     6421 acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc gtctcctcag
     6481 ggaagcctat cccgaaccct ctgttgggtc tcgatagtac caatggggcc gcaaccacga
     6541 cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gttgcagccc ctgtccctgc
     6601 gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg ctggacttcg
     6661 cctgtgattt ttgggtgctg gtggtggttg gtggagtcct ggcttgctat agcttgctag
     6721 taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc ctgcacagtg
     6781 actacatgaa catgactccc cgccgccccg ggcccacccg caagcattac cagccctatg
     6841 ccccaccacg cgacttcgca gcctatcgct ccgggggtac cgaattcagc gggtcaggcg
     6901 gatctggaag tggtggtgga gaacttgatg aattggtata cttactagat gggccaggtt
     6961 atgaccctat acattgcgat gtagtgacaa ggggcggcag ccaccttttc aattttggaa
     7021 gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac
     7081 ctatgattca tcttggtcac atcctcttct tgctgctgtt gccagttgct gccgcacaaa
     7141 caaccccagg tgagaggtca agtcttcccg ccttctatcc gggcacaagc gggagttgta
     7201 gtgggtgtgg atctttgtcc cttcctatat acatctgggc tcctctggct ggcacttgcg
     7261 gagtgcttct gctgagtctg gttattaccg gttctgttgt tattgttggt agaattattt
     7321 tatctggtag tggtagtatc acggcctact cccaacagac gcggggccta cttggttgca
     7381 tcatcactag cctcacaggc cgggacaaga accaggtcga aggggaggtt caagtggttt
     7441 ctaccgcaac acaatctttc ctggcgacct gcgtcaacgg cgtgtgctgg actgtctacc
     7501 atggcgctgg ctcgaagacc ctagccggtc caaaaggtcc aatcacccaa atgtacacca
     7561 atgtagacca ggacctcgtc ggctggcagg cgcctccagg ggcgcgctcc ttgacaccat
     7621 gcacctgtgg cagctcggac ctttacttgg tcacgagaca tgctgatgtc attccggtgc
     7681 gccggcgagg cgacagcagg ggaagtctac tctcccccag gcccgtctcc tacctgaaag
     7741 gctccgcagg tggtccattg ctttgccctt cggggcacgc tgtgggcatc ttccgggctg
     7801 ctgtgtgcac ccggggggtc gcgaaggcgg tggacttcgt gcccgttgag tctatggaaa
     7861 ctaccatgcg gtctagagtg aagttcagca ggagcgcaga cgcccccgcg tacaagcagg
     7921 gccagaacca gctctataac gagctcaatc taggacgaag agaggagtac gatgttttgg
     7981 acaagagacg tggccgggac cctgagatgg ggggaaagcc gagaaggaag aaccctcagg
     8041 aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt gagattggga
     8101 tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt ctcagtacag
     8161 ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc ggcatggtga
     8221 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     8281 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     8341 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     8401 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     8461 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     8521 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     8581 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     8641 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     8701 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     8761 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     8821 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     8881 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     8941 agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
     9001 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     9061 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     9121 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     9181 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     9241 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     9301 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     9361 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     9421 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     9481 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     9541 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta
     9601 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9661 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9721 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9901 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     9961 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
    10021 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
    10081 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
    10141 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
    10201 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
    10261 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
    10321 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
    10381 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
    10441 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
    10501 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    10561 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    10621 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
    10681 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    10741 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10981 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    11041 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    11101 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11701 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    12001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    12061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12661 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
    12721 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    12781 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    12841 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc c
//